Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue

Figure 4

Catalase knockdown increases palmitate-induced inflammation in bone marrow-derived macrophages. Bone marrow-derived macrophages from WT and CKO mice were treated with 500 μM PA for 6 h, using BSA as control. (a) JNK phosphorylation was measured by western blot (upper panel) and densitometry (lower panel). (b) IL-1β, (c) IL-6, and (d) TNF-α mRNA expression was measured by real-time PCR. Data are mean ± SE of four experiments. versus WT macrophages exposed to BSA; versus CKO macrophages exposed to BSA; versus WT macrophages exposed to PA.

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