Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue

Figure 6

Pharmacological or genetic inhibition of catalase alters macrophage activation in response to LPS or IL-4. RAW264.7 cells were treated with 0, 1, 2, 5, and 10 mM 3-AT and stimulated with 1 ng/mL LPS for 6 h or 24 h. (a) JNK phosphorylation at 6 h and (b) iNOS levels at 24 h were quantified by western blot (upper panel) and densitometry (lower panel). versus control; versus LPS. (c) JNK phosphorylation at 6 h and (d) iNOS abundance at 24 h were also measured by western blot (upper panel) and densitometry (lower panel) in WT and CKO bone marrow-derived macrophages treated with 1 ng/mL LPS for 6 h or 24 h. versus unstimulated WT macrophages; versus unstimulated CKO macrophages; versus WT macrophages exposed to LPS. All data are mean ± SE of four experiments. (e) CD206 and (f) arginase-1 mRNA levels were measured in RAW264.7 cells treated with 0, 2, and 5 mM 3-AT 1 h prior to stimulation with 10 ng/mL IL-4 for 6 h. versus control; versus IL-4. (g) CD206 and (h) arginase-1 mRNA levels were also measured in WT and CKO bone marrow-derived macrophages treated with 10 ng/mL IL-4 for 6 h. versus unstimulated WT macrophages; versus unstimulated CKO macrophages; versus WT macrophages exposed to IL-4. Data are from real-time PCR and are reported as mean ± SE of four experiments.

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