Effect of High, Medium, and Low Molecular Weight Hyaluronan on Inflammation and Oxidative Stress in an In Vitro Model of Human Nasal Epithelial Cells

Figure 5

Effect of U0126, HMW-HA, MMW-HA, and LMW-HA on nuclear translocation of NF-B in RPMI 2650 cells stimulated with rhIL-17A. The cells were preincubated (a) with U0126 (25 μM) or (b) with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 30 min; NF-B was evaluated in nuclear cell lysate by western blot. The bars represent ratio of band intensity and β-actin of 3 separate experiments. Representative gel images of NF-B and β-actin are shown. ANOVA with Fisher’s test correction was used for the analysis of the data. was statistically significant.

(a)

(b)