Inhibitory Effect of Loranthus parasiticus on IgE-Mediated Allergic Responses in RBL-2H3 Cells

Figure 1

Effect of LPE on degranulation and cell viability in IgE-mediated RBL-2H3 cells. RBL-2H3 cells were seeded on a 24-well plate (1 × 10⁵ cells/well) or a 96-well plate (1 × 10⁴ cells/well) in MEM-α with 10% FBS at 37°C overnight and further incubated with DNP-IgE for 24 h. IgE-sensitized cells were preincubated with LPE (0 to 400 μg/mL) for 1 h and then stimulated with DNP-HSA (0.1 μg/mL) for 4 h. β-Hexosaminidase activity and cell viability were determined as described in Section 2. Data are the mean ± SD values of triple or octuple determinations. versus DNP-HSA-treated group. (a) β-Hexosaminidase; (b) cell viability.

(a)

(b)