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Mediators of Inflammation
Volume 2016 (2016), Article ID 8970291, 9 pages
Research Article

Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function

1Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, Poland
2Department of Vascular, General and Transplant Surgery, Wroclaw Medical University, 52-129 Wrocław, Poland
3Department of Histology and Embryology, Wroclaw Medical University, 50-368 Wrocław, Poland

Received 29 February 2016; Revised 22 April 2016; Accepted 9 May 2016

Academic Editor: Simi Ali

Copyright © 2016 Dorota Kamińska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Renal transplant candidates present immune dysregulation, caused by chronic uremia. The aim of the study was to investigate whether pretransplant peripheral blood gene expression of immune factors affects clinical outcome of renal allograft recipients. Methods. In a prospective study, we analyzed pretransplant peripheral blood gene expression in87 renal transplant candidates with real-time PCR on custom-designed low density arrays (TaqMan). Results. Immediate posttransplant graft function (14-day GFR) was influenced negatively by TGFB1 () and positively by IL-2 gene expression (). Pretransplant blood mRNA expression of apoptosis-related genes (CASP3, FAS, and IL-18) and Th1-derived cytokine gene IFNG correlated positively with short- (6-month GFR CASP3: , FAS: , and IFNG: ) and long-term graft function (24-month GFR CASP3: , FAS: , IL-18: , and IFNG: ). Conclusion. Lowered pretransplant Th1-derived cytokine and apoptosis-related gene expressions were a hallmark of subsequent worse kidney function but not of acute rejection rate. The pretransplant IFNG and CASP3 and FAS and IL-18 genes’ expression in the recipients’ peripheral blood is the possible candidate for novel biomarker of short- and long-term allograft function.