Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells

Figure 1

rhIL-17A increased ChAT protein expression and mRNA in 16-HBE cells. Cells were stimulated with rhIL-17A (0–50 ng/mL) for 24 h to evaluate ChAT protein expression (a) by flow cytometry. Bars represent mean ± SD of fluorescence mean intensity (FMI) of three separate experiments. Representative (b) flow cytometry analysis and western blot (c) are shown. Bars represent mean ± SD of arbitrary densitometric units (ADU). Representative western blot analysis of ChAT protein and β-actin is shown. (d) Cells were stimulated with rhIL-17A (0–20 ng/mL) for 24 h to evaluate ChAT mRNA levels by RT-PCR. Bars represent mean ± SD of arbitrary units of three separate experiments and were plotted as fold change compared to untreated cells. Statistical analysis was performed by ANOVA test followed by Fisher’s PLSD multiple comparison test or Student’s -test. The black curve represents the anti-IgG isotype negative control antibody.

(a)

(b)

(c)

(d)