Autocrine Acetylcholine, Induced by IL-17A via NF<i>κ</i>B and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells

<div>rhIL-17A increased endogenous ACh binding and production in 16-HBE cells. Cells were stimulated with rhIL-17A (0–50 ng/mL) for 24 h to evaluate (a) ACh binding by flow cytometry. Bars represent mean ± SD of fluorescence mean intensity (FMI) of three separate experiments. (b) Representative flow cytometry analysis is shown. (c) ACh production expressed as pmoli/<i>μ</i>g protein. Bars represent mean ± SD of three different experiments. Statistical analysis was performed by ANOVA test followed by Fisher’s PLSD multiple comparison test. The black curve represents the anti-IgG isotype negative control antibody.</div>

Mediators of Inflammation

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Figure 2

Figure 2: Autocrine Acetylcholine, Induced by IL-17A via NF<i>κ</i>B and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells 

Figure 2 | Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells 