Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells

Figure 3

rhIL-17A increased pIkBα and pERK1/2 in 16-HBE cells. Cells were stimulated with rhIL-17A (0–20 ng/mL) for 30 min and 2 h to evaluate (a) pIkBα and (b) pERK1/2 by western blot. Cells were stimulated with rhIL-17A (0–50 ng/mL) for 30 min to evaluate (c) pIkBα and (d) pERK1/2 by western blot. Bars represent the mean ± SD of arbitrary densitometric units (ADU) of three separate experiments. Representative western blot analyses of pIkBα, pERK1/2 protein, and β-actin are shown. (e) NFκB-p65 subunit in nuclear and cytosol extracts from 16-HBE cells stimulated with rhIL-17A 20 ng/mL. Western blot analysis is shown. Statistical analysis was performed by Student’s -test and by ANOVA test followed by Fisher’s PLSD multiple comparison test.