Autocrine Acetylcholine, Induced by IL-17A via NF<i>κ</i>B and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells

<div>Effects of PD098,059 (25 <i>μ</i>M) and Bay11-7082 (50 <i>μ</i>M) on ChAT protein expression in 16-HBE cells. Cells were preincubated for 1 h with PD098,059 (25 <i>μ</i>M) or Bay11-7082 (50 <i>μ</i>M) and then stimulated with rhIL-17A 20 ng/mL for 24 h to evaluate ChAT protein expression (a) by flow cytometry. Bars represent mean ± SD of fluorescence mean intensity (FMI) of three separate experiments. Representative (b) flow cytometry analysis and western blot (c) are shown. Bars represent mean ± SD of arbitrary densitometric units (ADU). Representative western blot is shown. Statistical analysis was performed by ANOVA test followed by Fisher’s PLSD multiple comparison test. The black curve represents the anti-IgG isotype negative control antibody.</div>

Mediators of Inflammation

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Figure 4

Figure 4: Autocrine Acetylcholine, Induced by IL-17A via NF<i>κ</i>B and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells 

Figure 4 | Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells 