Autocrine Acetylcholine, Induced by IL-17A via NF<i>κ</i>B and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells

<div>Effect of Hemicholinium-3 (HCh-3) or Tiotropium on IL-8 production in 16-HBE cells. Cells were preincubated for 1 h with HCh-3 (50 <i>μ</i>M) or Tiotropium (100 nM) and then stimulated with rhIL-17A 20 ng/mL for 24 h to evaluate ((a)-(b)) IL-8 expression by western blot. Bars represent mean ± SD of arbitrary densitometric units (ADU). Representative western blot analyses of IL-8 protein and <i>β</i>-actin are shown. (c) IL-8 release (pg/mL, by ELISA). The values shown are the mean ± SD of three separate experiments. Statistical analysis was performed by ANOVA test followed by Fisher’s PLSD multiple comparison test.</div>

Mediators of Inflammation

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Figure 6

Figure 6: Autocrine Acetylcholine, Induced by IL-17A via NF<i>κ</i>B and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells 

Figure 6 | Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells 