Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK1/2 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells

Figure 9

Effect of Tiotropium on ChAT, Muc5AC, and IL-8 in N-HBE cells. Cells were incubated for 1 h with Tiotropium (100 nM) before addition of rhIL-17A 20 ng/mL for 24 h to evaluate (a) ChAT protein expression by western blot. Bars represent mean ± SD of arbitrary densitometric units (ADU). Representative western blot analysis of ChAT protein and β-actin is shown. (b) Muc5AC protein expression by flow cytometry. Bars represent mean ± SD of fluorescence mean intensity (FMI) of three separate experiments and were plotted as fold change compared to untreated cells. (c) Muc5AC protein expression by western blot. Bars represent mean ± SD of arbitrary densitometric units (ADU) of three different experiments. Representative western blot analysis of Muc5AC protein is shown. (d) IL-8 expression by western blot. Bars represent mean ± SD of arbitrary densitometric units (ADU). Representative western blot analyses of IL-8 protein and β-actin are shown. Statistical analysis was performed by ANOVA followed by Fisher’s PLSD multiple comparison test. was accepted as statistically significant.

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