Uncoupling Protein 2 Increases Susceptibility to Lipopolysaccharide-Induced Acute Lung Injury in Mice

Figure 1

Regulation of UCP2 expression by UCP2 recombinant adenovirus or genipin in mouse lungs. (a) Western blot analysis detected UCP2 expression in lung homogenates of mice after intranasal administration of saline, UCP2-NC, or different UCP2-Ad virus doses (1 × 10⁸, 5 × 10⁸, or 1 × 10⁹ pfu/mouse). (b) Western blot analysis detected UCP2 expression in lung at 3, 5, and 7 days after administration of saline, UCP2-NC, or UCP2-Ad virus at a dose of 5 × 10⁸ pfu/mouse. (c) Immunohistochemical analysis of UCP2 overexpression with an antibody specific to mouse UCP2 (brown color) in the control group treated with UCP2-NC virus (control) and the UCP2-Ad-treated group (400x). (d, e) Densitometric analysis of UCP2 immunoreactive bands is shown as fold difference in UCP2 expression normalized to actin expression (, versus the saline group (control)). (f, g) Western blot analysis detected the role of genipin in UCP2 expression in lung tissue (, versus the saline group, versus the LPS group, versus the UCP2-Ad + LPS group). Values are presented as means ± SEM.

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