Uncoupling Protein 2 Increases Susceptibility to Lipopolysaccharide-Induced Acute Lung Injury in Mice

Figure 3

Effects of UCP2 on mitochondrial ATP levels, membrane potential, and ROS production in lung tissue. (a) ATP levels were measured by a luciferase-based assay in isolated mitochondria. (b) The membrane potential (Δψm) of isolated mitochondria (1 mg/mL) was measured using Rhodamine 123 (1 mM). (c) Lipid peroxidation (MDA assay) were measured in the lung tissues of mice ( versus UCP2-NC + saline group, versus UCP2-NC + LPS group, and versus UCP2-Ad + LPS group). Values are presented as means ± SEM ().

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