Cross-Linking Mast Cell Specific Gangliosides Stimulates the Release of Newly Formed Lipid Mediators and Newly Synthesized Cytokines

Figure 5

Cross-linking GD1b derived gangliosides by mAbAA4 induced the activation of the transcription factors NFκB and NFAT. NFκB2 cells, VB9 cells, IC2 cells, and IH10 cells were sensitized with IgE anti-TNP and stimulated with DNP₄₈-HSA (50 ng/mL) for 1 h and rinsed and cultured for additional 5 h ((a); NFκB) or 15 h ((b); NFAT). For FcεRI independent stimulation, the cells were incubated with calcium ionophore (0.1 μg/mL). To cross-link GD1b derived gangliosides, cells were incubated with mAbAA4 (1, 2.5, 5, and 10 μg/mL). GFP expression was analyzed by flow cytometry. Data is expressed as the mean ± SD of three independent experiments. between experimental samples and the nonstimulated (NS) cells. between experimental samples and FcεRI stimulated cells.

(a)

(b)