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Mediators of Inflammation
Volume 2016 (2016), Article ID 9348037, 8 pages
http://dx.doi.org/10.1155/2016/9348037
Research Article

Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats

1Department of Burn and Plastic Surgery, Guizhou Provincial People’s Hospital, Guiyang 550002, China
2Institute of Neuroscience, Kunming Medical University, Kunming 650031, China
3Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China
4Department of Pediatrics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China
5Experimental Animal Center, Kunming Medical University, Kunming 650031, China

Received 21 September 2015; Revised 27 December 2015; Accepted 29 December 2015

Academic Editor: Mirella Giovarelli

Copyright © 2016 De-Yi Zheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Acute lung injury (ALI) induced by intestinal ischemia/reperfusion (II/R) has high incidence and mortality, in which IL-1β was essential for the full development of ALI. However, the detailed regulating mechanism for this phenomenon remains to be unclear. The purpose of this study was to investigate whether inhibition of P38 MAPK could downregulate the expression of IL-1β to protect lung from acute injury in II/R rats. Here, we found that the level of pulmonary edema at 16 hours after operation (hpo) was obviously enhanced compared to that in 8hpo and sham groups. Immunofluorescent staining demonstrated that IL-1β and P38 MAPK were detected in lung tissues. And rats with II/R have the highest translation level for IL-1β and phosphorylation of P38 MAPK in lung tissues at 16hpo compared with 8hpo and sham groups. Moreover, administration of SB239063, an inhibitor of P38 α and β, could effectively downregulate the expressions of IL-1β and protects lung tissues from injury in II/R rats. Our findings indicate that the inhibition of P38 α and β may downregulate the expression of IL-1β to protect lung from acute injury in II/R, which could be used as a potential target for reducing ALI induced by II/R in the future clinical trial.