Effect of 4-Allyl-1-hydroxy-2-methoxybenzene (Eugenol) on Inflammatory and Apoptosis Processes in Dental Pulp Fibroblasts

Table 1

Primers and conditions employed for evaluation of gene expression.

Gene symbol	Primer sequence (5′-3′)	Annealing temperature (°C)	Size of PCR product (bp)
Apaf-1	Fw: ATGAGGCTCTAGACGAAGCCATGT	55.7	490
	Rv: TGAATCAGATGAGCAGGGCCTACA
P53	Fw: TTGCTATCTGGGACAGCCAAGT	59.1	491
	Rv: CAGGCACAAACATGCACCTCAAAG
TNF-α	Fw: CGTCTCCTACCAGACCAAGGTCAA	58.8	401
	Rv: TTCCTGAATCCCAGGTTTCGAAGTG
NF-κB	Fw: CCTTGCACTTGGCAGTGATCACTA	59.3	294
	Rv: ACTTCTGCTCCTGAGCATTGACG
VEGFA	Fw: ATCACCGAGCCCGGAAGATTAGA	55	384
	Rv: CGGTGTTCCCAAAACTGGGTCAT
GAPDH	Fw: CCATCAATGACCCCTTCATTGACC	65.2	435
	Rv: TGGTCATGAGTCCTTCCACGAT
IL1-β	Fw: CGATGCACCTGTACGATCACTGAA	56.9	223
	Rv: CAACACGCAGGACAGGTACAGATT

Apaf-1-apoptotic peptidase activation factor 1; P53-tumor suppressor P53; TNF-α-tumor necrosis factor-alfa; NF-κB-nuclear factor kappa B; VEGFA-vascular endothelial growth factor A; GAPDH-glyceraldehyde 3-phosphate dehydrogenase; IL-1β-interleukin 1 beta.