Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

Figure 1

NHBE cells isolated from COPD donors have enhanced ER stress responses compared to nonsmokers and smokers. (a) Fully differentiated NHBE cells from nonsmoking individuals () exposed to room air (RA) or cigarette smoke (CS) from 4 cigarettes every second day (3 exposures) using a Vitrocell VC-10 smoking robot. LDH release into media and IL-6 gene expression were examined. (b) Gene expression of ATF4, XBP1, GRP78, GRP94, EDEM1, and CHOP was examined. (c) Fully differentiated NHBE cells from nonsmoker (NS), smoker (SM), and COPD (COPD) individuals ( donors per group) were examined for gene expression of ATF4, XBP1, GRP78, GRP94, EDEM1, and CHOP. Dot plots are represented as relative quantification (RQ) compared to ACTB expression and shown as the mean ± SEM, where each measurement was performed on 3 independent days on 6 donors/group. denotes value < 0.05, when comparing both treatments connected by a line, determined by Student’s -test (2 groups) or 2-way ANOVA with Tukey’s post hoc test (>2 groups).

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