Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

Figure 3

Reintroducing GPx-1 into NHBE cells isolated from COPD donors subdues the UPR. (a) Gene expression of GPx-1 was determined in fully differentiated NHBE cells from nonsmoking individuals () exposed to room air (RA) and cigarette smoke (CS; from 4 cigarettes every second day (3 exposures)) using a Vitrocell VC-10 smoking robot (b) RNA and (c) protein was analyzed for GPx-1 expression from fully differentiated NHBE cells from nonsmoker (NS), smokers, (SM) and COPD (COPD) individuals ( donors per group). (d) NHBE cells isolated from COPD subjects were transfected with albumin or GPx-1 protein and CHOP expression was determined by qPCR. (e) Immunoblots and corresponding densitometry analysis for CHOP, Gpx-1, and β-actin from NHBE cells from COPD subjects following albumin or GPx-1 protein transfection. In each immunoblot, every lane represents an individual cell donor. Data are shown as mean ± SEM, where each measurement was performed on 3 independent days on 6 donors/group. denotes a value < 0.05, when comparing both treatments connected by a line, determined by Student’s -test (2 groups) or 2-way ANOVA with Tukey’s post hoc test (>2 groups).

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