Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

Figure 6

Gpx-1 deficient mice have heightened ER stress in their lungs following exposure to cigarette smoke. Gpx-1^−/− and wild type mice were exposed to cigarette smoke daily for 1 year. (a) TUNEL analysis was performed on lung tissue from each mouse group. (b) Enhanced lung tissue caspase-3 cleavage coincided with (c) elevated LDH into BALF of Gpx-1^−/− exposed to cigarette smoke. Every lane in (b) represents an individual mouse and densitometry units (DU) of pixel intensity expressed as a ratio to total caspase-3 levels. Dot plots are represented as mean ± SEM, where each measurement was performed on 3 independent days on 6 donors/group. denotes a value < 0.05, when comparing both treatments connected by a line, determined by 2-way ANOVA with Tukey’s post hoc test (>2 groups).

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