Significant Contribution of Mouse Mast Cell Protease 4 in Early Phases of Experimental Autoimmune Encephalomyelitis

Figure 3

(a) Photomicrograph of the spinal cords stained with GFAP or Iba1. White and grey matters are delimited by the red line. Scale bar is 200 μm. Upper panels: GFAP marking in (i) WT mice, 2 weeks after EAE, or (ii) mMCP-4 KO mice, 2 weeks after EAE. Lower panel: Iba1 staining in (iii) WT mice, 2 weeks after EAE, or (iv) mMCP-4 KO mice, 2 weeks after EAE. (b) Grey matter levels of microglia and astrocytes are decreased in mMCP-4 KO mice 2 weeks after EAE. Left panel, percentage of astrocytes in 2 weeks post-EAE-induced mice; right panel, percentage of microglia 2 weeks after EAE. or for EAE-induced WT versus EAE-induced mMCP-4 KO mice. Each bar represents the mean ± SEM in 4 mice, in duplicate.

(a)

(b)