Identification of a Novel lincRNA-p21-miR-181b-PTEN Signaling Cascade in Liver Fibrosis
Effects of lincRNA-p21 overexpression on cell proliferation, α-SMA, type I collagen, and PTEN in LX-2 cells. Cells were transduced with Ad-lincRNA-p21 for 48 h and treated with PTEN siRNA for additional 48 h. (a) lincRNA-p21 was detected in cells transduced with Ad-lincRNA-p21. Overexpression of lincRNA-p21 suppressed cell proliferation (b), α-SMA mRNA (c), α-SMA protein (d), Col1A1 mRNA (e), and type I collagen (f), which were almost blocked down by PTEN siRNA. Cell proliferation was assessed by CCK-8 assay. PTEN mRNA (g) and protein (h) expressions were upregulated by Ad-lincRNA-p21. GAPDH was used as internal control. Each value is the mean ± SD of three experiments. compared with the control and compared with Ad-lincRNA-p21 group.
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