Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells

Figure 1

AdOSM induces IL-33 expression in vivo. Mice were culled after 7 days of an endotracheal administration of AdDel70 or AdOSM vectors ( for each treatment) and lung tissue was prepared for protein or RNA analysis as described in Materials and Methods. (a) Lung homogenate protein samples of BALB/c mice (lanes 1–5: individual mice treated with AdDel70; lanes 6–10: individual mice treated with AdOSM) were probed for IL-33, pSTAT3, total STAT3, and actin as indicated. (b, c) Quantification of band intensity was completed as described in Materials and Methods and white bars represent samples from BALB/c mice and black bars represent samples from C57BL/6 mice treated in parallel. IL-33 levels were normalized to actin; pSTAT3 was normalized to total STAT3. (d, e) RNA from total lung was probed by qRT-PCR for IL-33 or the IL-33 receptor chain ST2. The results represent the mean ± SEM, and statistical significance is indicated, where () or (). These data are representative of at least two experiments with identical trends. BLUeye molecular weight marker migration is shown on the right of panel (a).

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