Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells

Figure 3

C10 lung epithelial cell IL-33 responses to OSM in vitro. C10 alveolar epithelial cells were stimulated with the indicated cytokines (5 ng/mL) and lysed after 2, 4, and 6 hours. C10s were also stimulated with varying concentrations of recombinant OSM and lysed after 24 hours. (a) Cell lysates were probed for IL-33, pSTAT3, and actin protein as indicated. (b, c) Quantification of bands was completed using Image Studio Lite, where IL-33 was corrected for actin and pSTAT3 was corrected for total STAT3. (d, e) IL-33 and ST2 mRNA expression in C10 cells stimulated with or without 5 ng/mL OSM for the indicated times as assessed by qRT-PCR (C: control/unstimulated). The results represent the mean ± SEM. BLUeye molecular weight marker migration is shown on the right of panel (a).

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