Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells

Figure 4

STAT inhibition reduces IL-33 induction in vitro. (a) C10 alveolar epithelial cells were treated with the indicated inhibitors prior to stimulation with recombinant OSM (5 ng/mL) and lysed at 6 hours (Veh.: vehicle; PD: PD98059; SB: SB203580; AKTX: AKT inhibitor; Stat.: Stattic). Cell lysates were probed for IL-33 protein. (b) Immunoblot band intensities for IL-33 were quantified and corrected for actin as in Materials and Methods. (c) C10 cells were inhibited with varying concentrations of Stattic for 30 minutes prior to stimulation with OSM. Cells were lysed after 20 minutes, and lysates were probed for pSTAT3, phospho-p38, and actin, as indicated. BLUeye molecular weight marker migration is shown on the right of panels (a) and (c).

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