Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells

Figure 5

Cytokine modulation of OSM-induced IL-33 in vitro. C10 alveolar epithelial cells were stimulated with the indicated cytokines (5 ng/mL) and lysed after 24 hours. (a) Cell lysates were probed for IL-33 and actin as indicated (upper panel) and quantified as previously indicated (lower panel). (b) IL-33, pSTAT3, SMAD proteins, and actin protein expression levels were assessed by immunoblots of lysates from C10 cells stimulated with the indicated cytokines (all at 5 ng/mL). IL-33 band intensity was quantified as described in Materials and Methods (lower panel of (b)). BLUeye molecular weight marker migration is shown on the right of panels (a) and (b).

(a)

(b)