Proliferative Effects of Histamine on Primary Human Pterygium Fibroblasts

Figure 5

Histamine also showed proliferative effect on HCFs but at a higher effective concentration than on HPFs. (a) DNA agarose gel electrophoresis showed the expression of H1R, H2R, and H4R on HCFs, and the expression of H2R and H4R was quite few. (b) RT-qPCR showed that the expression of H1R in HCFs was only half of those in HPFs. (c) MTT assay showed that histamine had proliferative effect on HCFs in 0%, 5%, and 10% FBS situation at concentration of 100 μmol/L. compared to 0 histamine and 0% FBS, compared to 0 histamine and 5% FBS, compared to 0 histamine and 10% FBS. (d) MTT assay showed that H1R antagonist could efficiently inhibit the proliferative effect of histamine. compared to incubation with 100 μmol/L of histamine alone. compared to incubation without histamine and antagonist.

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