Apoptosis of Endothelial Cells by 13-HPODE Contributes to Impairment of Endothelial Barrier Integrity
Mean changes in BMEC mRNA expression of oxylipid biosynthetic enzymes (a, b), adhesion molecules (c, d), and a chemotactic cytokine (e). Media controls are displayed as open bars. Positive control is 4 hr lipopolysaccharide (LPS) and displayed in closed bars. Light grey bars represent S. uberis supernatant exposure for 4 hr and 36 hr. The mRNA expression is expressed as SE. Asterisks () denote differences between media control and treatments including S. uberis and positive control, LPS, as tested by Student’s t-tests. Significance declared for differences in ΔCt at .05 ( for all genes).