Apoptosis of Endothelial Cells by 13-HPODE Contributes to Impairment of Endothelial Barrier Integrity

Table 1

Bovine primers for qRT-PCR.

Gene	Reference sequence	TaqMan assay ID
TBP	NM_001075742.1	Bt03241946_m1
PGK1	NM_001034299.1	Bt03225857_m1
ACTB	NM_173979.3	Bt03279174_g1
ICAM-1	NM_174348.2	Bt03213911_m1
VCAM-1	NM_174484.1	Bt03279189_m1
IL-6	NM_173923.2	Bt03211905_m1
IL-8	NM_173925.2	Bt03211906_m1
IL-10	NM_174088.1	Bt03212727_m1
iNOS	M_001076799.1	Bt03249581_m1
15-LOX-1	NM_174501.2	Bt03214775_m1
COX-2	NM_174445.2	Bt03214492_m1

TBP: TATA-box binding protein; PGK1: phosphoglycerate kinase; ACTB: β-actin; ICAM-1: intercellular adhesion molecule-1; VCAM-1: vascular adhesion molecule-1; IL: interleukin, iNOS: inducible nitric oxide synthase; 15-LOX-1: 15-lipoxygenase-1; COX-2: cyclooxygenase-2.
Applied Biosystems Foster City, CA.