Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint Disease

Figure 4

Effects of PRGF on TNF-α pretreated synoviocyte cytokine release. 5% and 10% PRGF was added to TNF-α pretreated K4IM media for 6 h. After twice wash step with PBS, the medium was replaced by serum-starved media for 24 h. The release of endogenous cytokines was measured by ELISA. (a) Addition of 10% PRGF to the media of TNF-α pretreated (+TNF-α) K4IM and untreated cells led to significant reduction of endogenous TNF-α release. (b) IL-6 protein level is reduced in +TNF-α K4IM at a PRGF concentration of 10%. PRGF has no effect on IL-6 release in untreated cells. (c) The endogenous release of IL-1β is reduced when 5% PRGF were added to prestimulated K4IM. 10% PRGF has no effect on IL-1β release in untreated or prestimulated cells. (d) 10% PRGF in the medium of both untreated K4IM and TNF-α prestimulated K4IM increases the IL-10 protein release in media. (e) 5% and 10% PRGF in the medium of TNF-α prestimulated K4IM resulted in an increased concentration of VEGF, . Significant versus the control group without TNF-α prestimulation and without PRGF. ^#Significant versus group with TNF-α and without PRGF, .

(a)

(b)

(c)

(d)

(e)