Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint Disease

Figure 5

Effects of PRGF on TNF-α pretreated synoviocyte cytokine expression. To confirm the effect of PRGF on gene expression of inflammatory mediators, real-time RT-PCR was performed. (a) The endogenous expression of TNF-α is reduced when 5% and 10% PRGF are added to the medium. (b) Addition of 10% PRGF increases the IL-6 gene expression in untreated cells but has no significant effect on TNF-α prestimulated cells. (c) IL-1β gene expression is attenuated when 10% PRGF was added, . Significant versus the control group without TNF-α prestimulation and without PRGF. ^#Significant versus group with TNF-α and without PRGF, .

(a)

(b)

(c)