ZNF395 Is an Activator of a Subset of IFN-Stimulated Genes

Figure 2

ZNF395 modulates the IFNα-mediated induction of ISGs. (a) QRT-PCR with RNA from RTS3b cells that have been transfected with pooled siRNA against ZNF395 or control siRNA, and treated with 1000 U/ml IFNα for 6 h as indicated. The RT-PCR results for MX2, TRIM22, and IFIT2 are shown. (b) The RTS3b-TR-FLAG-ZNF395-1 cell line, allowing the Dox-inducible overexpression of ZNF395, was treated with IFNα. The fold changes of the factors in response to the addition of Dox compared to minus Dox, which was set as 1 in all cases, are given. The expression of ZNF395 was monitored by qRT-PCR in RTS3b cells after transfection with pooled siRNA against ZNF395 or siControl and in the RTS3b-TR-FLAG-ZNF395-1 line, in the presence and absence of Dox. In all cases, the CP-values have been normalized against those for the house keeping gene HPRT. Fold changes were calculated by Δ-threshold method [40]. RT-PCR results are the means of three to six independent assays. Standard deviations are shown.

(a)

(b)