ZNF395 Is an Activator of a Subset of IFN-Stimulated Genes

Figure 3

ZNF395 modulates the expression of CXCL10 and CXCL11. Primary human epithelial keratinocytes (NHEK), RTS3b cells, and primary human foreskin fibroblasts (HFF) were transfected with siRNA against ZNF395 or control siRNA and treated with IFNα or IFNγ, as indicated. (a) QRT-PCR was performed with primers to amplify CXCL10 and HPRT. The fold changes after normalization for the expression of HPRT were calculated and adjusted to the value of nontreated NHEK transfected with control siRNA, which was defined as 1. The result of ELISA-assays with the supernatant of NHEK and HFF to determine the concentrations of CXCL10 is shown on the right. (b) CXCL11 expression analyzed by qRT-PCR with RNA from siRNA transfected NHEK, RTS3b, and HFF treated with either IFNα or γ. (c) The relative expression level of ZNF395 in siControl transfected NHEK was set as 1 and the fold changes of all other cell types have been calculated. The diagrams show the means of three independent experiments. Standard deviations are given.

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