ZNF395 Is an Activator of a Subset of IFN-Stimulated Genes

Figure 5

ZNF395 requires intact NFκB motifs to enhance CXCL10 expression. RTS3b cells were cotransfected with the wt CXCL10 promoter-luciferase reporter construct or CXCL10 promoter-luciferase reporter constructs containing mutations within the binding sites of the indicated transcription factors and either 7.5 pmol siRNA against ZNF395 or the same amount of control siRNA. 48 h later the firefly luciferase activity was determined and normalized against the protein concentration of each sample. The relative luciferase activity of the wt CXCL10 promoter in the presence of siControl was set as 1 and the fold activations of the mutated constructs after cotransfection of control siRNA or siRNA against ZNF395 were calculated. The graph represents the average of 6 independent assays. The standard deviations are given. The values calculated by the -test for paired samples are given.