Research Article

Endogenous IL-33 Deficiency Exacerbates Liver Injury and Increases Hepatic Influx of Neutrophils in Acute Murine Viral Hepatitis

Figure 1

Transcript and protein expression of IL-33 in the liver of L2-MHV3-infected WT mice. (a) Quantification of IL-33mRNA expression by RT-qPCR in the liver of WT control mice (PBS) and 48 h PI and 72 h PI L2-MHV3 infection. The results were normalized to 18S expression and expressed as a fold change compared to the noninfected (PBS) condition. (b) Total liver protein of WT mice control (PBS) or from WT L2-MHV3-infected mice 48 h or 72 h PI were analyzed by western blotting for IL-33 expression. (c) IL-33 immunostaining of paraffin-embedded mouse liver control (PBS) or infected with L2-MHV3 for 48 h or 72 h (arrows indicate IL-33-stained hepatocyte nuclei). (d) Counting of IL-33-stained nuclei in paraffin-embedded liver immunostained for IL-33 from WT mice control (PBS) or infected with L2-MHV3 for 48 h or 72 h. (e) Quantification of IL-33 mRNA induction by RT-qPCR in primary mouse hepatocytes (PMH) infected in vitro with L2-MHV3 (MOI 1). The basal expression of IL-33 in PMH before infection (day 0) was used as a control and arbitrarily considered as 1 AU (arbitrary unit) which served as a reference for fold change in other conditions. Statistical analyses were performed according to Student’s t-test. ; .