Endogenous IL-33 Deficiency Exacerbates Liver Injury and Increases Hepatic Influx of Neutrophils in Acute Murine Viral Hepatitis

Figure 6

Comparative mRNA expression of neutrophil recruitment-associated chemokines in L2-MHV3-infected WT and IL-33 KO mice. Total liver RNA was extracted from WT or IL-33 KO mice and control (PBS) or L2-MHV3-infected mice (48 h and 72 h PI) and tested by RT-qPCR for (a) CXCR1, (b) CXCR2, (c) CXCL1, (d) CXCL2, (e) CXCL3, (f) CXCL5, (g) CCL2, and (h) CCL6. The mean expression of the WT PBS mice was used as a control and arbitrarily considered as 1 AU (arbitrary unit) which served as a reference for fold change in other conditions. Statistical analyses were done according to the Mann–Whitney U test. # represents significant difference with WT PBS mice, represents significant difference between WT and IL-33 KO mice, and $ represents significant difference between two conditions in the same background mice. ^#; ^##; and ^###.

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