Chemokines (CCL3, CCL4, and CCL5) Inhibit ATP-Induced Release of IL-1β by Monocytic Cells

Figure 1

CCL chemokines inhibit ATP-induced IL-1β release. (a) Human monocytic U937 cells were primed with LPS (1 μg/ml, 5 h) and activated with 2′(3′)-O-(4-benzoylbenzoyl)adenosine-5′-triphosphate (BzATP; 100 μM, 30 min). The release of IL-1β to the cell culture supernatant was measured by ELISA. Chemokines CCL3 (a), CCL4 (b), and CCL5 (c) dose dependently and efficiently inhibited the BzATP-induced release of IL-1β. In contrast, chemokine CXCL16 (d) was ineffective. Data are presented as individual data points, bars indicate median, and whiskers percentiles are 25 and 75; ; , in comparison to cells treated with LPS and BzATP.

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