Activated Platelets Induce an Anti-Inflammatory Response of Monocytes/Macrophages through Cross-Regulation of PGE2 and Cytokines
Collagen-activated platelets selectively regulate the synthesis of IL-10 and TNFα in cocultures with murine macrophages. (a), (b) Murine bone marrow-derived macrophages (BMDM) were incubated alone, with untreated platelets (untreated PLTs) or with platelets prestimulated with collagen (1 μg/ml; act. PLTs) for 3 hours and the concentrations of IL-10 and TNFα were determined by ELISA. (c) Murine BMDM were incubated with untreated platelets with or without murine IL-10 (125 pg/ml). TNFα levels were determined in the medium. Data are presented as mean ± SEM from 4 experiments. One-way ANOVA/Bonferroni , , , .