Activated Platelets Induce an Anti-Inflammatory Response of Monocytes/Macrophages through Cross-Regulation of PGE₂ and Cytokines

Figure 5

Ex vivo validation of the cross-regulation between platelet PGE₂ and IL-10 and TNFα released from EP2- and EP4-deficient macrophages. Murine BMDM from wild-type, EP2-deficient, and EP4-deficient mice were incubated alone, with untreated platelets (untreated PLTs) or with platelets prestimulated with collagen (1 μg/ml; act. PLTs) for 3 hours. The concentrations of PGE₂ (a) were determined by LC-MS/MS. IL-10 levels (b) and TNFα levels (c) were determined by ELISA. One-way ANOVA/Bonferroni , , , , ^#< 0.00008.

(a)

(b)

(c)