Activated Platelets Induce an Anti-Inflammatory Response of Monocytes/Macrophages through Cross-Regulation of PGE₂ and Cytokines

Figure 6

PGE₂ mediates inhibition of TNFα release from platelet-stimulated human monocytes. (a), (b), (c) Human monocytes were incubated alone, with untreated human platelets or with collagen-activated human platelets for 3 hours and PGE₂ (a), TNFα (b), or IL-10 (c) were determined. (d), (e) Human monocytes were incubated alone or with untreated platelets with or without PGE₂ (1 μM). (f) Human monocytes were incubated with untreated platelets with or without human IL-10 (125 pg/ml). The concentrations of PGE₂, TNFα, and IL-10 within the cell-free supernatants were determined by LC-MS/MS or rather ELISA within cell-free supernatants. Data are presented as mean ± SEM from 4 experiments. One-way ANOVA/Bonferroni (a)–(e) or two-tailed t-test (f) , , .

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