Syk Plays a Critical Role in the Expression and Activation of IRAK1 in LPS-Treated Macrophages

Figure 2

Syk impacts the regulation of IRAK1 activation in LPS-treated RAW264.7 cells. (a and c) Total or phospho-protein levels of IRAK1, Syk, Myc, TAK1, and β-actin as determined by lysate immunoblotting prepared from LPS- (1 μg/ml) treated RAW264.7-WT, RAW264.7-Syk^−/− cells, or RAW264.7-Syk^−/− cells transfected for 24 h with Myc-tagged Syk (5 × 10⁶ cells/ml). (b) Syk mRNA levels from RAW264.7-WT or RAW264.7-Syk^−/− cells treated with LPS (1 μg/ml) as determined using real-time PCR. All data are expressed as the mean ± SD of three independent replicate experiments.

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