IL-34 Upregulated Th17 Production through Increased IL-6 Expression by Rheumatoid Fibroblast-Like Synoviocytes

Figure 2

The interaction of IL-34 and CSF-1R promoted the production of IL-6 by RA FLS. (a) RA FLS () were stimulated with IL-34 (50 ng/ml) for 24 h. IL-6 mRNA expression was measured by RT-PCR (left); data represents the mean ± SEM, compared to the control group (middle). RA FLS () were incubated with 50 ng/ml IL-34 for 0, 12, 24, 48 and 72 h. IL-6 levels in the supernatants were measured by ELISA. Data represents the mean ± SEM, (right). (b) Cultured RA FLS () were stained with APC-labeled anti-CSF-1R (5 μg/ml) or isotype control antibody (5 μg/ml). CSF-1R expression was detected by flow cytometric analysis. Data represents the mean ± SEM. compared with the isotype control antibody group. (c) RA FLS () were stimulated with IL-34 (50 ng/ml) for 0, 12, 24, 48, and 72 h after 30 min preincubation with 25 ng/ml anti-CSF-1R mAb or not. Data represents the mean ± SEM. compared with the group incubated with anti-CSF-1R mAb.

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