Involvement of Toll Like Receptor 2 Signaling in Secondary Injury during Experimental Diffuse Axonal Injury in Rats

Figure 6

Role of TLR2 on secondary neuronal and axonal injury after DAI. (a) To confirm the role of TLR2 on the secondary neuronal injury, immumohistochemical staining of β-APP, tau, NF-L, and H&E staining in the rat cortex was performed (scale bar = 100 μm). (b) The bar graphs showed the statistical results for β-APP, tau, and NF-L assessed by immunohistochemistry. The relative changes in β-APP, tau, and NF-L levels were counted in 10 random fields of relevant region (40x magnification) and assessed by densitometry (IOD). (c) The expression of β-APP and tau in each group was determined by western blotting. β-Actin was used as an internal control. (d) The bar graphs showed the statistical results for β-APP and tau expression. Data were presented as mean ± SD in three separate experiments ( = 6; < 0.05, compared with control group; < 0.05, compared with DAI 3 d group).

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