Involvement of Toll Like Receptor 2 Signaling in Secondary Injury during Experimental Diffuse Axonal Injury in Rats

Figure 8

TLR2 signaling was related to cell apoptosis after DAI. TUNEL assay was used to detect apoptotic cells in rat cortex. (a) Colocalization of TUNEL (green) and nuclei (DAPI, blue) were considered as apoptotic cells (scale bar = 100 μm; enlarged figures scale bar = 20 μm). (b) The bar graphs showed the statistical results of apoptotic cells accounts. The positive cell levels were counted in 10 random fields of relevant region (40x magnification). Data were presented as mean ± SD in three separate experiments ( = 6; < 0.05, compared with control group; < 0.05, compared with DAI 3 d group). Apoptotic index (AI), defined as the number of TUNEL positive cells per mm².

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