The Astrocytic S100B Protein with Its Receptor RAGE Is Aberrantly Expressed in SOD1^G93A Models, and Its Inhibition Decreases the Expression of Proinflammatory Genes

Figure 6

Intracellular and extracellular increases of S100B by transfection of SOD1^G93A in C6 cells. C6 cells were transiently transfected with pCMV (mock), pCMV-SOD1^wild-type (SOD1^wt), or pCMV-SOD1^G93A (SOD1^G93A). (a) Cells were lysed and analyzed by Western blotting with anti-S100B, anti-RAGE, and anti-SOD1. The lower panel shows signal quantifications expressed in arbitrary units (AU), relative to mock, and reported as mean ± s.d. ( independent experiments). . (b) ELISA assay for S100B contained in the supernatant of C6 cells transfected with mock, SOD1^wt, or SOD1^G93A plasmids. Mean ± s.d. ( independent experiments). .

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