SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells
High glucose significantly activated the NF-κB inflammatory signaling by degradation of IκBα. GMCs were treated with 30 mmol/L high glucose for 6, 12, 24, 48, and 72 h (a) or the indicated concentrations of glucose or mannitol (b) for 24 h; the expression of p-IκBα, IκBα, p-NF-κB, and NF-κB in lysates of cells was detected by Western blotting. Data were normalized with respect to β-actin and are expressed as mean ± SD (). The gray graphs confirmed these trends. MCP-1 and IL-6 protein levels in the cell culture supernatant were determined by ELISA-based quantification (c). compared with the NC group, # compared with the 30 mmol/L high glucose stimulation group.
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