Mediators of Inflammation / 2017 / Article / Fig 4

Research Article

SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells

Figure 4

High glucose-mediated activation of NF-κB inflammatory signaling was reversed by siRNA-PIASy. (a) Western blotting analysis of protein expression after siRNA-mediated knockdown of PIASy has proven effective in silencing target genes of PIASy by siRNA-PIASy-3. (b) Immunoprecipitation and immunoblot analysis of the interaction between IKKγ and SUMO1 or SUMO2/3 induced by high glucose was reversed by siRNA-PIASy-3. (c) Western blotting analysis showed that the changes in PIASy, p-IKKγ, p-IκBα, IκBα, p-NF-κBp65, and NF-κBp65 expression after high glucose challenge were significantly reversed by siRNA-PIASy-3. (d) ELISA-based quantification indicated that high glucose-induced release of MCP-1 and IL-6 from GMCs was blunted by siRNA-PIASy-3. The results are presented as mean ± SD (); the gray graphs confirmed these trends. compared with the NC group, # compared with the 30 mmol/L high glucose stimulation group.

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