Evidence for the Involvement of Lipid Rafts and Plasma Membrane Sphingolipid Hydrolases in Pseudomonas aeruginosa Infection of Cystic Fibrosis Bronchial Epithelial Cells

Figure 10

Radioactivity distribution within gradient fractions of CuFi-1 cells infected or not with P. aeruginosa. Cell sphingolipids were labeled at the steady state with [1-³H]-sphingosine. We isolated the lipid rafts from infected and noninfected cells as the detergent-resistant membrane (DRM) following the procedure described in details in “Materials and Methods”. After ultracentrifugation, we collected eleven fractions and we measured the radioactivity associated to each of them.