The Combination of MBP and BCG-Induced Dendritic Cell Maturation through TLR2/TLR4 Promotes Th1 Activation In Vitro and Vivo

Figure 6

The direct and indirect effect of TLR2 and TLR4 on the production of cytokines in CD4⁺ T cells cocultured with DCs treated with the combination of MBP and BCG. (a) The function of TLR2/TLR4 expressed on DCs in the proliferation of CD4⁺ T cells from normal mice. CD4⁺ T cells from normal mice cocultured with DCs form TLR2^−/− mice or TLR4^−/− mice were stimulated with the combination of MBP and BCG for 48 h. The proliferation of CD4⁺ T cells was assayed with the CCK8 method. Data are expressed as the mean ± standard deviation and are shown in a bar graph. (b) Spots were counted by an ELISPOT reader. (c) ELISPOT cells showing IFN-γ production by CD4⁺ T cells cocultured with DCs from different groups after incubation with the combination of MBP and BCG. (d) ELISPOT cells showing IL-4 production by CD4⁺ T cells cocultured with DCs from different groups after incubation with the combination of MBP and BCG. (e) ELISA was used to detect the production of IL-10 in CD4⁺T cells cocultured with DCs from different groups treatment with the combination of MBP and BCG. is considered to indicate statistically significant differences compared with the coculture of CD4⁺ T cells from WT mice with DC cells from WT mice. compared with coculture of CD4⁺T cells from TLR2^−/− mice with DC cells from WT mice.

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