The Combination of MBP and BCG-Induced Dendritic Cell Maturation through TLR2/TLR4 Promotes Th1 Activation In Vitro and Vivo

Figure 9

The effect of TLR2/TLR4 expression on the activation of CD4⁺ T cells induced by the combination of MBP and BCG in vivo. (a) CCK8 assays were used to analyze the proliferation of CD4⁺ T cells from different types of mice immunized with MBP, BCG, or the combination of MBP and BCG. (b) CD4⁺ T cells were isolated from WT mice, TLR2^−/− mice, or TLR4^−/− mice immunized with MBP, BCG, or the combination of MBP and BCG, respectively. The mRNA levels of IFN-γ in the CD4⁺ T cells from these groups were measured with RT-PCR. is considered to indicate statistically significant differences compared with the NS group derived from WT mice. , compared with BCG group from different types of mice.

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