Bisphenol A Does Not Mimic Estrogen in the Promotion of the In Vitro Response of Murine Dendritic Cells to Toll-Like Receptor Ligands

Figure 1

Estrogen enhances the differentiation of cDCs while BPA does not affect cDCs. (a–d) Days 6-7 of culture of cDCs grown in the absence or presence of low (0.05 nM) and high doses (50 nM) of 17beta-estradiol (E2) or low (0.05 nM) and high doses (50 nM) of BPA. (a) Representative plot of the flow cytometry analysis of the cDC differentiation markers CD11c and CD11b expression in the alive gate of cDCs. (b) MFI of CD11c expression. (c) MFI of CD11b expression, at days 6-7 of culture. (d) Results of manual cell counts of cells alive in the same DC cultures in which dead cells positive for the trypan blue staining were excluded; the results are averages and SE of four experiments conducted with four independent BMDC cultures. represents , for , and for .

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